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Title: Genetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins
Authors: Khoo, G. 
Loh, E.Y.F.
Lim, T.M. 
Phang, V.P.E. 
Keywords: Isoelectric focusing
Sarcoplasmic proteins
Siamese fighting fish (Betta splendens)
Issue Date: 1997
Citation: Khoo, G.,Loh, E.Y.F.,Lim, T.M.,Phang, V.P.E. (1997). Genetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins. Aquaculture International 5 (6) : 537-549. ScholarBank@NUS Repository.
Abstract: Sarcoplasmic proteins of four colour varieties of Siamese fighting fish, Betta splendens Regan, were analysed by isoelectric focusing (IEF). Genetic polymorphisms were detected in six loci having isoelectric points: 4.92-5.00 (Sp-1), 5.33-5.48 (Sp-2), 5.90 (Sp-3), 6.31-6.35 (Sp-4), 6.58-6.70 (Sp-5) and 6.90 (Sp-6). For the Sp-1, Sp-2, Sp-4 and Sp-5 loci, there were two co-dominant alleles. At Sp-3 and Sp-6, one allele was dominant and the other null. Individuals within each locus were classified into phenotypes A, AB and B. Only homozygotes were found at Sp-2. Allele frequencies were calculated for each locus and tested for accordance with the Hardy-Weinberg equilibrium. Observed values for each phenotype at Sp-1, Sp-3, Sp-4, Sp-5 and Sp-6 agreed well with the expected ones. At Sp-2, genotypic frequencies for each variety deviated markedly (p < 0.01) from the equilibrium. When tested for homogeneity, no significant differences (p ≥ 0.95) in gene frequencies were found among the varieties except at Sp-2. Band sharing index (BSI), computed using the BIO-GENE v5.04 program, revealed high intravariety genetic similarities (0.701-0.740) and slightly above-average intervariety values (0.550-0.583). In this study, we estimated the levels of genetic variability in B. splendens, and proposed a genetic model for each polymorphic locus.
Source Title: Aquaculture International
ISSN: 09676120
Appears in Collections:Staff Publications

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