Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100753
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dc.titleGenetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins
dc.contributor.authorKhoo, G.
dc.contributor.authorLoh, E.Y.F.
dc.contributor.authorLim, T.M.
dc.contributor.authorPhang, V.P.E.
dc.date.accessioned2014-10-27T08:29:20Z
dc.date.available2014-10-27T08:29:20Z
dc.date.issued1997
dc.identifier.citationKhoo, G.,Loh, E.Y.F.,Lim, T.M.,Phang, V.P.E. (1997). Genetic variation in different varieties of Siamese fighting fish using isoelectric focusing of sarcoplasmic proteins. Aquaculture International 5 (6) : 537-549. ScholarBank@NUS Repository.
dc.identifier.issn09676120
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100753
dc.description.abstractSarcoplasmic proteins of four colour varieties of Siamese fighting fish, Betta splendens Regan, were analysed by isoelectric focusing (IEF). Genetic polymorphisms were detected in six loci having isoelectric points: 4.92-5.00 (Sp-1), 5.33-5.48 (Sp-2), 5.90 (Sp-3), 6.31-6.35 (Sp-4), 6.58-6.70 (Sp-5) and 6.90 (Sp-6). For the Sp-1, Sp-2, Sp-4 and Sp-5 loci, there were two co-dominant alleles. At Sp-3 and Sp-6, one allele was dominant and the other null. Individuals within each locus were classified into phenotypes A, AB and B. Only homozygotes were found at Sp-2. Allele frequencies were calculated for each locus and tested for accordance with the Hardy-Weinberg equilibrium. Observed values for each phenotype at Sp-1, Sp-3, Sp-4, Sp-5 and Sp-6 agreed well with the expected ones. At Sp-2, genotypic frequencies for each variety deviated markedly (p < 0.01) from the equilibrium. When tested for homogeneity, no significant differences (p ≥ 0.95) in gene frequencies were found among the varieties except at Sp-2. Band sharing index (BSI), computed using the BIO-GENE v5.04 program, revealed high intravariety genetic similarities (0.701-0.740) and slightly above-average intervariety values (0.550-0.583). In this study, we estimated the levels of genetic variability in B. splendens, and proposed a genetic model for each polymorphic locus.
dc.sourceScopus
dc.subjectIsoelectric focusing
dc.subjectSarcoplasmic proteins
dc.subjectSiamese fighting fish (Betta splendens)
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleAquaculture International
dc.description.volume5
dc.description.issue6
dc.description.page537-549
dc.identifier.isiutNOT_IN_WOS
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