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Title: Ex-vivo NMR of unprocessed tissue in water: A simplified procedure for studying intracranial neoplasms
Authors: Xu, M.
Ye, J.
Yang, D. 
Xu, X.
Yeo, T.T.
Ng, W.H.
Lim, C.C.
Keywords: Brain neoplasm
High-resolution magic-angle spinning
Nuclear magnetic resonance
Rat brain
Issue Date: Dec-2007
Citation: Xu, M., Ye, J., Yang, D., Xu, X., Yeo, T.T., Ng, W.H., Lim, C.C. (2007-12). Ex-vivo NMR of unprocessed tissue in water: A simplified procedure for studying intracranial neoplasms. Analytical and Bioanalytical Chemistry 389 (7-8) : 2153-2159. ScholarBank@NUS Repository.
Abstract: Ex-vivo and in-vitro nuclear magnetic resonance (NMR) spectroscopy techniques have been used for studying chemical metabolites in surgically resected specimens of human neoplasms, and may provide complementary information to in-vivo whole-body magnetic-resonance spectroscopy (MRS). We describe an ex-vivo NMR in water method for measurement of water-soluble metabolites in unprocessed normal rat brain tissue and human intracranial neoplasms. The NMR spectra obtained using the method described here were comparable to those obtained using high-resolution magic-angle spinning (HRMAS) NMR methods, with good correlation in metabolite concentrations relative to creatine (r 2 = 0.7635). Improved spectral resolution and baseline were noted compared to HRMAS, but macromolecule resonances were not detected. Ex-vivo NMR of unprocessed tissue in water is rapid and technically simple to perform, and has the potential to be used for direct assessment of intracranial neoplasms. © 2007 Springer-Verlag.
Source Title: Analytical and Bioanalytical Chemistry
ISSN: 16182642
DOI: 10.1007/s00216-007-1629-9
Appears in Collections:Staff Publications

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