Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0308668101
Title: Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro
Authors: Hong, Y. 
Liu, T. 
Zhao, H. 
Xu, H. 
Wang, W.
Liu, R.
Chen, T. 
Deng, J.
Gui, J.
Keywords: Germ stem cells
Meiosis
Oryzias latipes
Spermiogenesis
Testis
Issue Date: 21-May-2004
Citation: Hong, Y., Liu, T., Zhao, H., Xu, H., Wang, W., Liu, R., Chen, T., Deng, J., Gui, J. (2004-05-21). Establishment of a normal medakafish spermatogonial cell line capable of sperm production in vitro. Proceedings of the National Academy of Sciences of the United States of America 101 (21) : 8011-8016. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0308668101
Abstract: Spermatogonia are the male germ stem cells that continuously produce sperm for the next generation. Spermatogenesis is a complicated process that proceeds through mitotic phase of stem cell renewal and differentiation, meiotic phase, and postmeiotic phase of spermiogenesis. Full recapitulation of spermatogenesis in vitro has been impossible, as generation of normal spermatogonial stem cell lines without immortalization and production of motile sperm from these cells after long-term culture have not been achieved. Here we report the derivation of a normal spermatogonial cell line from a mature medakafish testis without immortalization. After 140 passages during 2 years of culture, this cell line retains stable but growth factor-dependent proliferation, a diploid karyotype, and the phenotype and gene expression pattern of spermatogonial stem cells. Furthermore, we show that this cell line can undergo meiosis and spermiogenesis to generate motile sperm. Therefore, the ability of continuous proliferation and sperm production in culture is an intrinsic property of medaka spermatogonial stem cells, and immortalization apparently is not necessary to derive male germ cell cultures. Our findings and cell line will offer a unique opportunity to study and recapitulate spermatogenesis in vitro and to develop approaches for germ-line transmission.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
URI: http://scholarbank.nus.edu.sg/handle/10635/100606
ISSN: 00278424
DOI: 10.1073/pnas.0308668101
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