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https://doi.org/10.1107/S1744309107034239
Title: | Crystallization and preliminary X-ray diffraction analysis of hemextin A: A unique anticoagulant protein from Hemachatus haemachatus venom | Authors: | Banerjee, Y. Kumar, S. Jobichen, C. Kini, R.M. |
Keywords: | Anticoagulants Hemextin Snake-venom toxins Three-finger toxins |
Issue Date: | 28-Jul-2007 | Citation: | Banerjee, Y., Kumar, S., Jobichen, C., Kini, R.M. (2007-07-28). Crystallization and preliminary X-ray diffraction analysis of hemextin A: A unique anticoagulant protein from Hemachatus haemachatus venom. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 63 (8) : 701-703. ScholarBank@NUS Repository. https://doi.org/10.1107/S1744309107034239 | Abstract: | Hemextin A was isolated and purified from African Ringhals cobra (Hemachatus haemachatus). It is a three-finger toxin that specifically inhibits blood coagulation factor VIIa and clot formation and that also interacts with hemextin B to form a unique anticoagulant complex. Hemextin A was crystallized by the hanging-drop vapour-diffusion method by equilibration against 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6 and 30% PEG 4000 as the precipitating agent. The crystals belong to space group P212 121, with unit-cell parameters a = 49.27, b = 49.51, c = 57.87 Å and two molecules in the asymmetric unit. They diffracted to 1.5 Å resolution at beamline X25 at BNL. © International Union of Crystallography 2007. | Source Title: | Acta Crystallographica Section F: Structural Biology and Crystallization Communications | URI: | http://scholarbank.nus.edu.sg/handle/10635/100375 | ISSN: | 17443091 | DOI: | 10.1107/S1744309107034239 |
Appears in Collections: | Staff Publications |
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