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Title: Agrobacterium flagellar switch gene fliG is liquid inducible and important for virulence
Authors: Li, L.
Jia, Y.H.
Pan, S.Q. 
Keywords: Agrobacterium
Issue Date: 2002
Citation: Li, L., Jia, Y.H., Pan, S.Q. (2002). Agrobacterium flagellar switch gene fliG is liquid inducible and important for virulence. Canadian Journal of Microbiology 48 (8) : 753-758. ScholarBank@NUS Repository.
Abstract: Agrobacterium tumefaciens C58 was mutagenized with a mini-Tn5 transposon containing a promoterless gene encoding the green fluorescent protein (GFP). A mutant, CGS74, exhibited a higher GFP expression level in liquid media than on solid media. The ability of the mutant to cause tumors on plants was attenuated. Sequence analysis showed that the transposon was inserted at the fliG gene, which encodes a flagellar motor switch protein required for flagellar movement. Studies of the fliG-gfp fusion gene indicated that the promoter activity of the fliG gene was higher in liquid than in solid media. Electron microscopy studies demonstrated that the mutant was nonflagellate. This suggests that the A. tumefaciens motility is important for virulence and that bacterial flagellar synthesis occurs at a higher level in a liquid environment than in a solid environment, perhaps resulting in a higher motility.
Source Title: Canadian Journal of Microbiology
ISSN: 00084166
DOI: 10.1139/w02-067
Appears in Collections:Staff Publications

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