Please use this identifier to cite or link to this item: https://doi.org/10.1139/w02-067
Title: Agrobacterium flagellar switch gene fliG is liquid inducible and important for virulence
Authors: Li, L.
Jia, Y.H.
Pan, S.Q. 
Keywords: Agrobacterium
Flagella
FliG
Liquid
Virulence
Issue Date: 2002
Citation: Li, L., Jia, Y.H., Pan, S.Q. (2002). Agrobacterium flagellar switch gene fliG is liquid inducible and important for virulence. Canadian Journal of Microbiology 48 (8) : 753-758. ScholarBank@NUS Repository. https://doi.org/10.1139/w02-067
Abstract: Agrobacterium tumefaciens C58 was mutagenized with a mini-Tn5 transposon containing a promoterless gene encoding the green fluorescent protein (GFP). A mutant, CGS74, exhibited a higher GFP expression level in liquid media than on solid media. The ability of the mutant to cause tumors on plants was attenuated. Sequence analysis showed that the transposon was inserted at the fliG gene, which encodes a flagellar motor switch protein required for flagellar movement. Studies of the fliG-gfp fusion gene indicated that the promoter activity of the fliG gene was higher in liquid than in solid media. Electron microscopy studies demonstrated that the mutant was nonflagellate. This suggests that the A. tumefaciens motility is important for virulence and that bacterial flagellar synthesis occurs at a higher level in a liquid environment than in a solid environment, perhaps resulting in a higher motility.
Source Title: Canadian Journal of Microbiology
URI: http://scholarbank.nus.edu.sg/handle/10635/100024
ISSN: 00084166
DOI: 10.1139/w02-067
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

2
checked on Sep 17, 2018

WEB OF SCIENCETM
Citations

2
checked on Sep 17, 2018

Page view(s)

32
checked on Sep 7, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.