Please use this identifier to cite or link to this item: https://doi.org/10.1139/w02-067
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dc.titleAgrobacterium flagellar switch gene fliG is liquid inducible and important for virulence
dc.contributor.authorLi, L.
dc.contributor.authorJia, Y.H.
dc.contributor.authorPan, S.Q.
dc.date.accessioned2014-10-27T08:21:10Z
dc.date.available2014-10-27T08:21:10Z
dc.date.issued2002
dc.identifier.citationLi, L., Jia, Y.H., Pan, S.Q. (2002). Agrobacterium flagellar switch gene fliG is liquid inducible and important for virulence. Canadian Journal of Microbiology 48 (8) : 753-758. ScholarBank@NUS Repository. https://doi.org/10.1139/w02-067
dc.identifier.issn00084166
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100024
dc.description.abstractAgrobacterium tumefaciens C58 was mutagenized with a mini-Tn5 transposon containing a promoterless gene encoding the green fluorescent protein (GFP). A mutant, CGS74, exhibited a higher GFP expression level in liquid media than on solid media. The ability of the mutant to cause tumors on plants was attenuated. Sequence analysis showed that the transposon was inserted at the fliG gene, which encodes a flagellar motor switch protein required for flagellar movement. Studies of the fliG-gfp fusion gene indicated that the promoter activity of the fliG gene was higher in liquid than in solid media. Electron microscopy studies demonstrated that the mutant was nonflagellate. This suggests that the A. tumefaciens motility is important for virulence and that bacterial flagellar synthesis occurs at a higher level in a liquid environment than in a solid environment, perhaps resulting in a higher motility.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1139/w02-067
dc.sourceScopus
dc.subjectAgrobacterium
dc.subjectFlagella
dc.subjectFliG
dc.subjectLiquid
dc.subjectVirulence
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1139/w02-067
dc.description.sourcetitleCanadian Journal of Microbiology
dc.description.volume48
dc.description.issue8
dc.description.page753-758
dc.description.codenCJMIA
dc.identifier.isiut000178422500008
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