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|Title:||Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains||Authors:||Hoh, Y.K.
|Issue Date:||Oct-1992||Citation:||Hoh, Y.K., Tan, T.K., Yeoh, H.-H. (1992-10). Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains. Applied Biochemistry and Biotechnology 37 (1) : 81-88. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02788859||Abstract:||Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 × 10-2-9.1 × 10-2. After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and β-glucosidase activities. One segregant showed a 2.5-fold increase in β-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved β-glucosidase activities. © 1992 The Humana Press Inc.||Source Title:||Applied Biochemistry and Biotechnology||URI:||http://scholarbank.nus.edu.sg/handle/10635/99729||ISSN:||02732289||DOI:||10.1007/BF02788859|
|Appears in Collections:||Staff Publications|
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