Please use this identifier to cite or link to this item: https://doi.org/10.1007/BF02788859
Title: Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains
Authors: Hoh, Y.K.
Tan, T.K. 
Yeoh, H.-H. 
Keywords: β-glucosidase
Aspergillus niger
cellobiose hydrolysis
protoplast fusion
Issue Date: Oct-1992
Citation: Hoh, Y.K., Tan, T.K., Yeoh, H.-H. (1992-10). Protoplast Fusion of β-Glucosidase-Producing Aspergillus niger Strains. Applied Biochemistry and Biotechnology 37 (1) : 81-88. ScholarBank@NUS Repository. https://doi.org/10.1007/BF02788859
Abstract: Protoplast fusion, induced by polyethylene glycol and Ca2+, was carried out between two auxotrophic strains of Aspergillus niger. The fusion frequency ranged from 6.2 × 10-2-9.1 × 10-2. After induced haploidization of a diploid, various segregants showing combinations of the parental genetic markers were isolated. Unlike diploids, haploid segregants exhibited greater variations in their morphology and β-glucosidase activities. One segregant showed a 2.5-fold increase in β-glucosidase activity over those of the parents. Thus, this method appears promising for creating new recombinant strains of A. niger with improved β-glucosidase activities. © 1992 The Humana Press Inc.
Source Title: Applied Biochemistry and Biotechnology
URI: http://scholarbank.nus.edu.sg/handle/10635/99729
ISSN: 02732289
DOI: 10.1007/BF02788859
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