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Title: Therapeutic potential of peptides with neutralizing ability towards the venom and toxin (CaTx-I) of Crotalus adamanteus
Authors: Samy, R.P. ItemCrisRefDisplayStrategy.rp.deleted.icon
Thwin, M.M.
Stiles, B.G.
Bow, H.
Chow, V.T.K.
Gopalakrishnakone, P.
Keywords: Crotalus adamanteus
Phospholipase A 2
Phospholipase inhibitor
Issue Date: Oct-2011
Citation: Samy, R.P.,Thwin, M.M.,Stiles, B.G.,Bow, H.,Chow, V.T.K.,Gopalakrishnakone, P. (2011-10). Therapeutic potential of peptides with neutralizing ability towards the venom and toxin (CaTx-I) of Crotalus adamanteus. Current Topics in Medicinal Chemistry 11 (20) : 2540-2555. ScholarBank@NUS Repository.
Abstract: The CaTx-I (PLA 2) toxin of Crotalus adamanteus venom is responsible for most of the symptoms observed during envenomation. Synthetic peptides were designed and screened for venom (0.8 μg/ml) and CaTx-I (0.1 μM) inhibition at varying doses of the peptide (10000- 0.0001 μM) using a Cayman chemical human secretory phospholipase A 2 (sPLA 2, Type II) assay kit. Further, in vitro neutralization studies were evaluated by a fixed dose of peptide (1 μM) against venom (0.8 μg/ml) and toxin (0.1 μM). Among the linear peptides (PIP-18, cyclic C and PIP59-67) that showed potent neutralizing effects against the venom/toxin of C. adamanteus. PIP-18 [IC 50, 1.23 μM] and cyclic C [IC 50, 1.27 μM] peptides possessed the strongest inhibitory effect against CaTx-I. A fixed dose of CaTx-I (75 μg/kg) was administered intraperitoneally (i.p.) into mice followed by an i.p. injection of peptides PIP-18 and cyclic C at (6 μg/mouse), venom (150 μg/kg) and toxin CaTx-I alone served as references. Mice treated with PIP-18 and cyclic C showed a very strong neutralizing effect and markedly reduced mortality compared to the control after 24 h. The CA venom and CaTx-I injected mice showed severe toxicity after 24 h. Peptides PIP-18 and cyclic C were non-hemolytic at 100 μM. They produced a significant decrease in lipid peroxidase (LPx) and enhancement of superoxide dismutase (SOD), catalase (CAT) and Glutathione-s-transferase (GST) levels indicating their antioxidant property against venom-induced changes in mice. This study confirmed the potent snake venom neutralizing properties of peptides. © 2011 Bentham Science Publishers.
Source Title: Current Topics in Medicinal Chemistry
ISSN: 15680266
Appears in Collections:Staff Publications

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