Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/98344
Title: Therapeutic potential of peptides with neutralizing ability towards the venom and toxin (CaTx-I) of Crotalus adamanteus
Authors: Samy, R.P. 
Thwin, M.M.
Stiles, B.G.
Bow, H.
Chow, V.T.K.
Gopalakrishnakone, P.
Keywords: Crotalus adamanteus
Neutralization
Peptides
Phospholipase A 2
Phospholipase inhibitor
Snakebite
Issue Date: Oct-2011
Citation: Samy, R.P.,Thwin, M.M.,Stiles, B.G.,Bow, H.,Chow, V.T.K.,Gopalakrishnakone, P. (2011-10). Therapeutic potential of peptides with neutralizing ability towards the venom and toxin (CaTx-I) of Crotalus adamanteus. Current Topics in Medicinal Chemistry 11 (20) : 2540-2555. ScholarBank@NUS Repository.
Abstract: The CaTx-I (PLA 2) toxin of Crotalus adamanteus venom is responsible for most of the symptoms observed during envenomation. Synthetic peptides were designed and screened for venom (0.8 μg/ml) and CaTx-I (0.1 μM) inhibition at varying doses of the peptide (10000- 0.0001 μM) using a Cayman chemical human secretory phospholipase A 2 (sPLA 2, Type II) assay kit. Further, in vitro neutralization studies were evaluated by a fixed dose of peptide (1 μM) against venom (0.8 μg/ml) and toxin (0.1 μM). Among the linear peptides (PIP-18, cyclic C and PIP59-67) that showed potent neutralizing effects against the venom/toxin of C. adamanteus. PIP-18 [IC 50, 1.23 μM] and cyclic C [IC 50, 1.27 μM] peptides possessed the strongest inhibitory effect against CaTx-I. A fixed dose of CaTx-I (75 μg/kg) was administered intraperitoneally (i.p.) into mice followed by an i.p. injection of peptides PIP-18 and cyclic C at (6 μg/mouse), venom (150 μg/kg) and toxin CaTx-I alone served as references. Mice treated with PIP-18 and cyclic C showed a very strong neutralizing effect and markedly reduced mortality compared to the control after 24 h. The CA venom and CaTx-I injected mice showed severe toxicity after 24 h. Peptides PIP-18 and cyclic C were non-hemolytic at 100 μM. They produced a significant decrease in lipid peroxidase (LPx) and enhancement of superoxide dismutase (SOD), catalase (CAT) and Glutathione-s-transferase (GST) levels indicating their antioxidant property against venom-induced changes in mice. This study confirmed the potent snake venom neutralizing properties of peptides. © 2011 Bentham Science Publishers.
Source Title: Current Topics in Medicinal Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/98344
ISSN: 15680266
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

30
checked on Nov 16, 2018

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.