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Title: DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus
Authors: Liu, Y.
Wu, J. 
Chen, H.
Hew, C.L. 
Yan, J. 
Keywords: AFM
DNA condensation
Magnetic tweezers
Issue Date: 20-Dec-2010
Citation: Liu, Y., Wu, J., Chen, H., Hew, C.L., Yan, J. (2010-12-20). DNA condensates organized by the capsid protein VP15 in White Spot Syndrome Virus. Virology 408 (2) : 197-203. ScholarBank@NUS Repository.
Abstract: The White Spot Syndrome Virus (WSSV) has a large circular double-stranded DNA genome of around 300. kb and it replicates in the nucleus of the host cells. The machinery of how the viral DNA is packaged has been remained unclear. VP15, a highly basic protein, is one of the major capsid proteins found in the virus. Previously, it was shown to be a DNA binding protein and was hypothesized to participate in the viral DNA packaging process. Using Atomic Force Microscopy imaging, we show that the viral DNA is associated with a (or more) capsid proteins. The organized viral DNA qualitatively resembles the conformations of VP15 induced DNA condensates in vitro. Furthermore, single-DNA manipulation experiments revealed that VP15 is able to condense single DNA against forces of a few pico Newtons. Our results suggest that VP15 may aid in the viral DNA packaging process by directly condensing DNA. © 2010 Elsevier Inc.
Source Title: Virology
ISSN: 00426822
DOI: 10.1016/j.virol.2010.09.008
Appears in Collections:Staff Publications

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