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https://doi.org/10.1016/S0039-9140(97)00291-9
Title: | Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope | Authors: | Zhang, Y. Lee, H.K. Li, S.F.Y. |
Keywords: | Capillary electrophoresis Chip Epi-fluorescence microscope Short capillary |
Issue Date: | Feb-1998 | Citation: | Zhang, Y., Lee, H.K., Li, S.F.Y. (1998-02). Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope. Talanta 45 (4) : 613-618. ScholarBank@NUS Repository. https://doi.org/10.1016/S0039-9140(97)00291-9 | Abstract: | Fast, efficient separation of five free acid forms of porphyrins was achieved in a short capillary and a chip, respectively. The capillary was 6 cm long from injection end to detector with an electric field strength of 214 V cm-1. Separations were performed within 5 min. A glass microchip device was fabricated using standard photolithographic procedures and chemical wet etching. The channels were sealed using a direct bonding technique. For a separation length of 2.8 cm with electric field strength of 500 V cm, electrophoretic separations with baseline resolution were achieved in less than 2 min. A variable wavelength epi-fluorescence microscope was used as an on-column detector. | Source Title: | Talanta | URI: | http://scholarbank.nus.edu.sg/handle/10635/95465 | ISSN: | 00399140 | DOI: | 10.1016/S0039-9140(97)00291-9 |
Appears in Collections: | Staff Publications |
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