Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0039-9140(97)00291-9
Title: Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope
Authors: Zhang, Y.
Lee, H.K. 
Li, S.F.Y. 
Keywords: Capillary electrophoresis
Chip
Epi-fluorescence microscope
Short capillary
Issue Date: Feb-1998
Source: Zhang, Y., Lee, H.K., Li, S.F.Y. (1998-02). Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope. Talanta 45 (4) : 613-618. ScholarBank@NUS Repository. https://doi.org/10.1016/S0039-9140(97)00291-9
Abstract: Fast, efficient separation of five free acid forms of porphyrins was achieved in a short capillary and a chip, respectively. The capillary was 6 cm long from injection end to detector with an electric field strength of 214 V cm-1. Separations were performed within 5 min. A glass microchip device was fabricated using standard photolithographic procedures and chemical wet etching. The channels were sealed using a direct bonding technique. For a separation length of 2.8 cm with electric field strength of 500 V cm, electrophoretic separations with baseline resolution were achieved in less than 2 min. A variable wavelength epi-fluorescence microscope was used as an on-column detector.
Source Title: Talanta
URI: http://scholarbank.nus.edu.sg/handle/10635/95465
ISSN: 00399140
DOI: 10.1016/S0039-9140(97)00291-9
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