Please use this identifier to cite or link to this item:
|Title:||A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement||Authors:||Liu, T.
area under the curve (AUC)
|Issue Date:||28-Sep-2011||Citation:||Liu, T., Song, L., Wang, H., Huang, D. (2011-09-28). A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement. Journal of Agricultural and Food Chemistry 59 (18) : 9756-9762. ScholarBank@NUS Repository. https://doi.org/10.1021/jf202939d||Abstract:||A high-throughput method for rapid determination of starch hydrolase inhibition was developed using a 96-well microplate UV-vis reader to monitor the turbidity decrease over time. The area under the curve of turbidity measured over time was used to quantify the inhibitory effect of polyphenolic compounds on porcine pancreatic amylase, rat intestine α-glucosidase, and fungal amyloglucosidase. Acarbose equivalence (AE) was introduced for the first time and defined as IC 50 of acarbose divided by the IC 50 of the sample measured under the same 96-well plate. This way, the run-to-run variations are canceled out. Among the plant extracts tested, grape seed extracts (1,440 μmolAE/g) and cinnamon bark extracts (1600 μmolAE/g) are the most active in inhibiting rat intestine α-glucosidase. For porcine α-amylase inhibition, grape seed extracts (5710 μmol AE/g) are close to four times more active (equal weight basis) than acarbose (1550 μmolAE/g). © 2011 American Chemical Society.||Source Title:||Journal of Agricultural and Food Chemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/92949||ISSN:||00218561||DOI:||10.1021/jf202939d|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jun 17, 2019
WEB OF SCIENCETM
checked on Jun 10, 2019
checked on May 25, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.