Please use this identifier to cite or link to this item: https://doi.org/10.1021/jf202939d
Title: A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement
Authors: Liu, T.
Song, L.
Wang, H.
Huang, D. 
Keywords: α-Amylase
amyloglucosidase
area under the curve (AUC)
proanthocyanidins
R-glucosidase
starch hydrolysis
turbidity
Issue Date: 28-Sep-2011
Source: Liu, T., Song, L., Wang, H., Huang, D. (2011-09-28). A high-throughput assay for quantification of starch hydrolase inhibition based on turbidity measurement. Journal of Agricultural and Food Chemistry 59 (18) : 9756-9762. ScholarBank@NUS Repository. https://doi.org/10.1021/jf202939d
Abstract: A high-throughput method for rapid determination of starch hydrolase inhibition was developed using a 96-well microplate UV-vis reader to monitor the turbidity decrease over time. The area under the curve of turbidity measured over time was used to quantify the inhibitory effect of polyphenolic compounds on porcine pancreatic amylase, rat intestine α-glucosidase, and fungal amyloglucosidase. Acarbose equivalence (AE) was introduced for the first time and defined as IC 50 of acarbose divided by the IC 50 of the sample measured under the same 96-well plate. This way, the run-to-run variations are canceled out. Among the plant extracts tested, grape seed extracts (1,440 μmolAE/g) and cinnamon bark extracts (1600 μmolAE/g) are the most active in inhibiting rat intestine α-glucosidase. For porcine α-amylase inhibition, grape seed extracts (5710 μmol AE/g) are close to four times more active (equal weight basis) than acarbose (1550 μmolAE/g). © 2011 American Chemical Society.
Source Title: Journal of Agricultural and Food Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/92949
ISSN: 00218561
DOI: 10.1021/jf202939d
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