Poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles prepared by high pressure homogenization for paclitaxel chemotherapy

Abstract

High pressure homogenization was employed in the current work to prepare poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles (NPs) for controlled release of paclitaxel. The prepared drug-loaded PLGA NPs were found of spherical shape with a size of 200-300 nm. The drug encapsulation efficiency ranged from 34.8 ± 1.6 to 62.6 ± 7.9% depending on the homogenization pressure and cycles. Paclitaxel was released from the nanoparticles in a biphasic profile with a fast release rate in the first 3 days followed by a slow first-order release. A higher or comparable cytotoxicity against glioma C6 cells was found for the drug formulated in the PLGA NPs in comparison with the free drug Taxol®. Confocal laser scanning microscopy (CLSM) evidenced internalization of the fluorescent coumarin 6-loaded PLGA NPs by the C6 cells. The freeze-dried nanoparticles were found to possess excellent water redispersability. The high pressure homogenization could be applied for large industrial scale production of nanoparticles for drug delivery. © 2007 Elsevier B.V. All rights reserved.