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Title: An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida
Authors: Cheng, X.-Y.
Tong, Y.-W.
Loh, K.-C. 
Keywords: Immersed hollow fiber membrane bioreactor
Pharmaceuticals biotransformation
Pseudomonas putida
Substrate inhibition
Issue Date: 15-Jun-2014
Citation: Cheng, X.-Y., Tong, Y.-W., Loh, K.-C. (2014-06-15). An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida. Biochemical Engineering Journal 87 : 1-7. ScholarBank@NUS Repository.
Abstract: An immersed hollow fiber membrane bioreactor (IHFMB) was fabricated for production of pharmaceutical intermediates cis-indandiol from indene by Pseudomonas putida ATCC 55687. At 2-6g/L indene, significant increase of 160-400%, corresponding to 240-400mg/L of cis-indandiol was achieved as compared to that obtained from suspension culture bioreactors (SCB). The IHFMB volumetric productivity of 10.1-12.9mg/(Lh) and molar yield of 0.050-0.093mol/mol indene was also higher than the 4.3-8.3mg/(Lh) and 0.01-0.030mol/mol indene, respectively, in the SCB. The hollow fiber membranes in the IHFMB facilitated a more effective biotransformation through reactant sequestration and cell immobilization, with equal contribution at low indene concentrations, albeit a lower contribution by the latter at 6g/L indene. The performance of the IHFMB was further improved by process optimization and the highest product titer of 487mg/L, overall volumetric productivity of 24.6mg/(Lh) and molar yield of 0.126mol/mol indene were achieved with a module comprising 28 membranes (168cm2/150mL working volume) at a medium circulation rate of 5mL/min and aeration rate of 0.5vvm. Reusability of the membranes has also been successfully demonstrated through 5 successive operations of batch biotransformation. © 2014 Elsevier B.V.
Source Title: Biochemical Engineering Journal
ISSN: 1873295X
DOI: 10.1016/j.bej.2014.03.011
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