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https://doi.org/10.1016/j.bej.2014.03.011
DC Field | Value | |
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dc.title | An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida | |
dc.contributor.author | Cheng, X.-Y. | |
dc.contributor.author | Tong, Y.-W. | |
dc.contributor.author | Loh, K.-C. | |
dc.date.accessioned | 2014-10-09T06:43:21Z | |
dc.date.available | 2014-10-09T06:43:21Z | |
dc.date.issued | 2014-06-15 | |
dc.identifier.citation | Cheng, X.-Y., Tong, Y.-W., Loh, K.-C. (2014-06-15). An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida. Biochemical Engineering Journal 87 : 1-7. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bej.2014.03.011 | |
dc.identifier.issn | 1873295X | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/88531 | |
dc.description.abstract | An immersed hollow fiber membrane bioreactor (IHFMB) was fabricated for production of pharmaceutical intermediates cis-indandiol from indene by Pseudomonas putida ATCC 55687. At 2-6g/L indene, significant increase of 160-400%, corresponding to 240-400mg/L of cis-indandiol was achieved as compared to that obtained from suspension culture bioreactors (SCB). The IHFMB volumetric productivity of 10.1-12.9mg/(Lh) and molar yield of 0.050-0.093mol/mol indene was also higher than the 4.3-8.3mg/(Lh) and 0.01-0.030mol/mol indene, respectively, in the SCB. The hollow fiber membranes in the IHFMB facilitated a more effective biotransformation through reactant sequestration and cell immobilization, with equal contribution at low indene concentrations, albeit a lower contribution by the latter at 6g/L indene. The performance of the IHFMB was further improved by process optimization and the highest product titer of 487mg/L, overall volumetric productivity of 24.6mg/(Lh) and molar yield of 0.126mol/mol indene were achieved with a module comprising 28 membranes (168cm2/150mL working volume) at a medium circulation rate of 5mL/min and aeration rate of 0.5vvm. Reusability of the membranes has also been successfully demonstrated through 5 successive operations of batch biotransformation. © 2014 Elsevier B.V. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bej.2014.03.011 | |
dc.source | Scopus | |
dc.subject | Immersed hollow fiber membrane bioreactor | |
dc.subject | Pharmaceuticals biotransformation | |
dc.subject | Pseudomonas putida | |
dc.subject | Substrate inhibition | |
dc.type | Article | |
dc.contributor.department | CHEMICAL & BIOMOLECULAR ENGINEERING | |
dc.description.doi | 10.1016/j.bej.2014.03.011 | |
dc.description.sourcetitle | Biochemical Engineering Journal | |
dc.description.volume | 87 | |
dc.description.page | 1-7 | |
dc.description.coden | BEJOF | |
dc.identifier.isiut | 000336708300001 | |
Appears in Collections: | Staff Publications |
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