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Title: Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling
Authors: Lin, J.
Pan, S.
Zheng, W. 
Huang, Z. 
Issue Date: 21-Oct-2013
Citation: Lin, J., Pan, S., Zheng, W., Huang, Z. (2013-10-21). Polarization-resolved second-harmonic generation imaging for liver fibrosis assessment without labeling. Applied Physics Letters 103 (17) : -. ScholarBank@NUS Repository.
Abstract: We apply the polarization-resolved second-harmonic generation (PR-SHG) microscopy to investigate the changes of collagen typings (type I vs type III) and collagen fibril orientations of liver tissue in bile-duct-ligation (BDL) rat models. The PR-SHG results show that the second-order susceptibility tensor ratios (χ31/χ15 and χ33/ χ15) of collagen fibers increase with liver fibrotic progression after BDL surgery, reflecting an increase of the type III collagen component with the severity of liver fibrosis; and the square root of the collagen type III to type I ratio linearly correlates (R2 = 0.98) with histopathological scores. Furthermore, the collagen fibril orientations become more random with liver fibrosis transformation as compared to normal liver tissue. This work demonstrates that PR-SHG microscopy has the potential for label-free diagnosis and characterization of liver fibrosis based on quantitative analysis of collagen typings and fibril orientations. © 2013 AIP Publishing LLC.
Source Title: Applied Physics Letters
ISSN: 00036951
DOI: 10.1063/1.4826516
Appears in Collections:Staff Publications

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