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Title: Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells
Authors: Prabhakaran, M.P. 
Ghasemi-Mobarakeh, L.
Jin, G.
Ramakrishna, S. 
Keywords: Conducting polymer
Nerve tissue engineering
Neurite elongation
Issue Date: Nov-2011
Citation: Prabhakaran, M.P., Ghasemi-Mobarakeh, L., Jin, G., Ramakrishna, S. (2011-11). Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells. Journal of Bioscience and Bioengineering 112 (5) : 501-507. ScholarBank@NUS Repository.
Abstract: Tissue engineering of nerve grafts requires synergistic combination of scaffolds and techniques to promote and direct neurite outgrowth across the lesion for effective nerve regeneration. In this study, we fabricated a composite polymeric scaffold which is conductive in nature by electrospinning and further performed electrical stimulation of nerve stem cells seeded on the electrospun nanofibers. Poly-l-lactide (PLLA) was blended with polyaniline (PANi) at a ratio of 85:15 and electrospun to obtain PLLA/PANi nanofibers with fiber diameters of 195±30nm. The morphology, chemical and mechanical properties of the electrospun PLLA and PLLA/PANi scaffolds were carried out by scanning electron microscopy (SEM), X-ray photo electron spectroscopy (XPS) and tensile instrument. The electrospun PLLA/PANi fibers showed a conductance of 3×10 -9S by two-point probe measurement. In vitro electrical stimulation of the nerve stem cells cultured on PLLA/PANi scaffolds applied with an electric field of 100mV/mm for a period of 60min resulted in extended neurite outgrowth compared to the cells grown on non-stimulated scaffolds. Our studies further strengthen the implication of electrical stimulation of nerve stem cells on conducting polymeric scaffolds towards neurite elongation that could be effective for nerve tissue regeneration. © 2011 The Society for Biotechnology, Japan.
Source Title: Journal of Bioscience and Bioengineering
ISSN: 13891723
DOI: 10.1016/j.jbiosc.2011.07.010
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