Please use this identifier to cite or link to this item:
|Title:||Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells|
|Authors:||Prabhakaran, M.P. |
Nerve tissue engineering
|Citation:||Prabhakaran, M.P., Ghasemi-Mobarakeh, L., Jin, G., Ramakrishna, S. (2011-11). Electrospun conducting polymer nanofibers and electrical stimulation of nerve stem cells. Journal of Bioscience and Bioengineering 112 (5) : 501-507. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2011.07.010|
|Abstract:||Tissue engineering of nerve grafts requires synergistic combination of scaffolds and techniques to promote and direct neurite outgrowth across the lesion for effective nerve regeneration. In this study, we fabricated a composite polymeric scaffold which is conductive in nature by electrospinning and further performed electrical stimulation of nerve stem cells seeded on the electrospun nanofibers. Poly-l-lactide (PLLA) was blended with polyaniline (PANi) at a ratio of 85:15 and electrospun to obtain PLLA/PANi nanofibers with fiber diameters of 195±30nm. The morphology, chemical and mechanical properties of the electrospun PLLA and PLLA/PANi scaffolds were carried out by scanning electron microscopy (SEM), X-ray photo electron spectroscopy (XPS) and tensile instrument. The electrospun PLLA/PANi fibers showed a conductance of 3×10 -9S by two-point probe measurement. In vitro electrical stimulation of the nerve stem cells cultured on PLLA/PANi scaffolds applied with an electric field of 100mV/mm for a period of 60min resulted in extended neurite outgrowth compared to the cells grown on non-stimulated scaffolds. Our studies further strengthen the implication of electrical stimulation of nerve stem cells on conducting polymeric scaffolds towards neurite elongation that could be effective for nerve tissue regeneration. © 2011 The Society for Biotechnology, Japan.|
|Source Title:||Journal of Bioscience and Bioengineering|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 21, 2018
WEB OF SCIENCETM
checked on Apr 30, 2018
checked on May 4, 2018
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.