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https://doi.org/10.1016/S0021-9673(97)00667-5
Title: | Conventional capillary electrophoresis in comparison with short-capillary capillary electrophoresis and microfabricated glass chip capillary electrophoresis for the analysis of fluorescein isothiocyanate anti-human immunoglobulin C | Authors: | Rodriguez, I. Zhang, Y. Lee, H.K. Li, S.F.Y. |
Keywords: | Immunoglobulins Instrumentation Proteins |
Issue Date: | 26-Sep-1997 | Citation: | Rodriguez, I., Zhang, Y., Lee, H.K., Li, S.F.Y. (1997-09-26). Conventional capillary electrophoresis in comparison with short-capillary capillary electrophoresis and microfabricated glass chip capillary electrophoresis for the analysis of fluorescein isothiocyanate anti-human immunoglobulin C. Journal of Chromatography A 781 (1-2) : 287-293. ScholarBank@NUS Repository. https://doi.org/10.1016/S0021-9673(97)00667-5 | Abstract: | Fast, efficient analysis of fluorescein isothiocyanate anti-human IgG was achieved in a short-capillary and in a microfabricated glass chip. The capillary was 6 cm long from injection end to detector with electric field strength of 0.268 kV/cm. Analyses were performed within 1 min. A glass microchip device was fabricated using standard photolithographic procedures and chemical wet etching. The channels were seated using a direct bonding technique. For an effective length of 2.8 cm with electric field strength of 0.526 kV/cm, electrophoretic analysis was achieved in less than 16 s. Conventional capillary electrophoresis gave highly reproducible results and good detection limits. Analysis time was 2.5 min for a capillary with 47 cm effective length and electric field strength of 0.383 kV/cm. | Source Title: | Journal of Chromatography A | URI: | http://scholarbank.nus.edu.sg/handle/10635/77419 | ISSN: | 00219673 | DOI: | 10.1016/S0021-9673(97)00667-5 |
Appears in Collections: | Staff Publications |
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