Developing a strategy for activity-based detection of enzymes in a protein microarray

Abstract

Spot search: We have developed a microarray-based strategy for detection of three major classes of proteins on the basis of their enzymatic activities (see figure). This is the first protein array technology that allows detection of proteins not merely by their binding, but rather by their enzymatic activities. This strategy, together with increasing efforts in the field of protein based microarrays, should provide a valuable tool for screening and identification of new enzymes and their potential inhibitors in a high-throughput fashion.