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Title: Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis
Authors: Lin, J.
Lu, F. 
Zheng, W. 
Tai, D.C.S.
Yu, H. 
Sheppard, C. 
Huang, Z. 
Keywords: Coherent anti-stokes raman scattering
Fatty liver
Hepatic fibrosis
Multimodal nonlinear optical microscopy
Second harmonic generation
Two photon excitation fluorescence
Issue Date: 2011
Citation: Lin, J., Lu, F., Zheng, W., Tai, D.C.S., Yu, H., Sheppard, C., Huang, Z. (2011). Multimodal nonlinear optical imaging of obesity-induced liver steatosis and fibrosis. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 7903 : -. ScholarBank@NUS Repository.
Abstract: Liver steatosis/fibrosis represents the major conditions and symptoms for many liver diseases. Nonlinear optical microscopy has emerged as a powerful tool for label-free tissue imaging with high sensitivity and chemical specificity for several typical biochemical compounds. Three nonlinear microscopy imaging modalities are implemented on the sectioned tissues from diseased livers induced by high fat diet (HFD). Coherent anti-Stokes Raman scattering (CARS) imaging visualizes and quantifies the lipid droplets accumulated in the liver, Second harmonic generation (SHG) is used to map the distribution of aggregated collagen fibers, and two-photon excitation fluorescence (TPEF) reveals the morphology of hepatic cells based on the autofluorescence signals from NADH and flavins within the hepatocytes. Our results demonstrate that obesity induces liver steatosis in the beginning stage, which may progress into liver fibrosis with high risk. There is a certain correlation between liver steatosis and fibrosis. This study may provide new insights into the understanding of the mechanisms of steatosis/fibrosis transformations at the cellular and molecular levels. © 2011 SPIE.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ISBN: 9780819484406
ISSN: 16057422
DOI: 10.1117/12.873507
Appears in Collections:Staff Publications

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