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Title: An amperometric benzene sensor using whole cell Pseudomonas putida ML2
Authors: Tan, H.-M.
Cheong, S.-P. 
Tan, T.-C. 
Issue Date: 1994
Citation: Tan, H.-M., Cheong, S.-P., Tan, T.-C. (1994). An amperometric benzene sensor using whole cell Pseudomonas putida ML2. Biosensors and Bioelectronics 9 (1) : 1-8. ScholarBank@NUS Repository.
Abstract: Using a dissolved oxygen electrode and immobilized Pseudomonas putida ML2 whole cells, a biosensor was developed for the amperometric determination of benzene in aqueous solution. A linear relationship between the sensor response and the concentration of benzene was observed up to 1.35 mM in 50 mM phosphate buffer under the optimum conditions of pH 6.8 and at a temperature about 300K. Linear range sensitivity was 2.82 μA/mM. Maximum error was estimated to be about 3.55 μM. The steady state response time was about 2- 10 min within the linear range, comparable to other biosensors currently in use. The sensor response was stable, reproducible and highly specific for the sensing of benzene. With its specificity to benzene, rapid response and ease of operation, this whole cell P. putida ML2 benzene biosensor has potential commercial application for the monitoring and sensing of benzene in industrial waste effluents and in particular for monitoring benzene contamination in groundwater.
Source Title: Biosensors and Bioelectronics
ISSN: 09565663
DOI: 10.1016/0956-5663(94)80008-1
Appears in Collections:Staff Publications

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