Please use this identifier to cite or link to this item:
|Title:||Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser||Authors:||Lu, F.
|Issue Date:||2010||Citation:||Lu, F., Zheng, W., Lin, J., Huang, Z. (2010). Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser. Applied Physics Letters 96 (13) : -. ScholarBank@NUS Repository. https://doi.org/10.1063/1.3377905||Abstract:||We report on a unique implementation of a dual 4-f paired-gratings spectral filtering of a femtosecond (fs) laser to realize high contrast coherent anti-Stokes Raman scattering (CARS) microscopy and high quality multiphoton microscopy on the same platform for label-free biomolecular imaging. The 4-f gratings filtering enables readily conversion of the 100 fs laser pulse trains into transform-limited 1 picosecond (ps) pulses in the spectral range of 700-1600 nm for CARS imaging. Compared with fs-CARS microscopy, our ps-CARS imaging yields an approximately tenfold improvement in spectral resolution, while a twofold improvement in nonresonant background suppression. High quality second-harmonic generation and two-photon excitation fluorescence imaging can also be acquired in tandem when swapping the 4-f grating filtering from the ps mode to fs mode. We demonstrate this multimodal nonlinear optical microscopy technique by imaging 465 nm polystyrene beads in water and animal liver tissue. © 2010 American Institute of Physics.||Source Title:||Applied Physics Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/67120||ISSN:||00036951||DOI:||10.1063/1.3377905|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 15, 2019
WEB OF SCIENCETM
checked on Sep 6, 2019
checked on Sep 8, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.