Please use this identifier to cite or link to this item:
https://doi.org/10.1063/1.3377905
DC Field | Value | |
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dc.title | Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser | |
dc.contributor.author | Lu, F. | |
dc.contributor.author | Zheng, W. | |
dc.contributor.author | Lin, J. | |
dc.contributor.author | Huang, Z. | |
dc.date.accessioned | 2014-06-17T09:44:41Z | |
dc.date.available | 2014-06-17T09:44:41Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Lu, F., Zheng, W., Lin, J., Huang, Z. (2010). Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser. Applied Physics Letters 96 (13) : -. ScholarBank@NUS Repository. https://doi.org/10.1063/1.3377905 | |
dc.identifier.issn | 00036951 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/67120 | |
dc.description.abstract | We report on a unique implementation of a dual 4-f paired-gratings spectral filtering of a femtosecond (fs) laser to realize high contrast coherent anti-Stokes Raman scattering (CARS) microscopy and high quality multiphoton microscopy on the same platform for label-free biomolecular imaging. The 4-f gratings filtering enables readily conversion of the 100 fs laser pulse trains into transform-limited 1 picosecond (ps) pulses in the spectral range of 700-1600 nm for CARS imaging. Compared with fs-CARS microscopy, our ps-CARS imaging yields an approximately tenfold improvement in spectral resolution, while a twofold improvement in nonresonant background suppression. High quality second-harmonic generation and two-photon excitation fluorescence imaging can also be acquired in tandem when swapping the 4-f grating filtering from the ps mode to fs mode. We demonstrate this multimodal nonlinear optical microscopy technique by imaging 465 nm polystyrene beads in water and animal liver tissue. © 2010 American Institute of Physics. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1063/1.3377905 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | BIOENGINEERING | |
dc.description.doi | 10.1063/1.3377905 | |
dc.description.sourcetitle | Applied Physics Letters | |
dc.description.volume | 96 | |
dc.description.issue | 13 | |
dc.description.page | - | |
dc.description.coden | APPLA | |
dc.identifier.isiut | 000276275300080 | |
Appears in Collections: | Staff Publications |
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