Please use this identifier to cite or link to this item: https://doi.org/10.1063/1.3377905
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dc.titleIntegrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser
dc.contributor.authorLu, F.
dc.contributor.authorZheng, W.
dc.contributor.authorLin, J.
dc.contributor.authorHuang, Z.
dc.date.accessioned2014-06-17T09:44:41Z
dc.date.available2014-06-17T09:44:41Z
dc.date.issued2010
dc.identifier.citationLu, F., Zheng, W., Lin, J., Huang, Z. (2010). Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser. Applied Physics Letters 96 (13) : -. ScholarBank@NUS Repository. https://doi.org/10.1063/1.3377905
dc.identifier.issn00036951
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/67120
dc.description.abstractWe report on a unique implementation of a dual 4-f paired-gratings spectral filtering of a femtosecond (fs) laser to realize high contrast coherent anti-Stokes Raman scattering (CARS) microscopy and high quality multiphoton microscopy on the same platform for label-free biomolecular imaging. The 4-f gratings filtering enables readily conversion of the 100 fs laser pulse trains into transform-limited 1 picosecond (ps) pulses in the spectral range of 700-1600 nm for CARS imaging. Compared with fs-CARS microscopy, our ps-CARS imaging yields an approximately tenfold improvement in spectral resolution, while a twofold improvement in nonresonant background suppression. High quality second-harmonic generation and two-photon excitation fluorescence imaging can also be acquired in tandem when swapping the 4-f grating filtering from the ps mode to fs mode. We demonstrate this multimodal nonlinear optical microscopy technique by imaging 465 nm polystyrene beads in water and animal liver tissue. © 2010 American Institute of Physics.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1063/1.3377905
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.1063/1.3377905
dc.description.sourcetitleApplied Physics Letters
dc.description.volume96
dc.description.issue13
dc.description.page-
dc.description.codenAPPLA
dc.identifier.isiut000276275300080
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