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https://doi.org/10.1002/jbio.200900110
| Title: | Focal modulation microscopy with annular apertures: A numerical study | Authors: | Gong, W. Si, K. Chen, N. Sheppard, C.J.R. |
Keywords: | Annular apertures Background rejection Confocal microscopy Penetration depth Phase modulation Spatial resolution |
Issue Date: | Jul-2010 | Citation: | Gong, W., Si, K., Chen, N., Sheppard, C.J.R. (2010-07). Focal modulation microscopy with annular apertures: A numerical study. Journal of Biophotonics 3 (7) : 476-484. ScholarBank@NUS Repository. https://doi.org/10.1002/jbio.200900110 | Abstract: | Image formation of focal modulation microscopy with annular aperture (AFMM) is presented. The spatial resolution is discussed. Compared with confocal microscopy, AFMM can simultaneously enhance the axial and transverse resolution. By adjusting the width of the annular objective aperture, AFMM can be adjusted from best spatial resolution performance to highest signal level. The capability of background rejection is also investigated, showing AFMM has the potential to further increase the imaging penetration depth. Finally, an optimal configuration of AFMM is elaborated. Radial spoke pattern at the focal plane for focal modulation microscopy with annular apertures (AFMM). © 2010 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. | Source Title: | Journal of Biophotonics | URI: | http://scholarbank.nus.edu.sg/handle/10635/67066 | ISSN: | 1864063X | DOI: | 10.1002/jbio.200900110 |
| Appears in Collections: | Staff Publications |
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