Please use this identifier to cite or link to this item:
|dc.title||Recent advances in biomolecular process intensification|
|dc.identifier.citation||Choe, W.-S., Nian, R., Lai, W.-B. (2006). Recent advances in biomolecular process intensification. Chemical Engineering Science 61 (3) : 886-906. ScholarBank@NUS Repository.|
|dc.description.abstract||Escherichia coli has been the most popular cellular biofactory for the production of many biomolecules including recombinant proteins whose bioactivities are not dependent on complicated post-translational modifications such as glycosylation. Despite many advantages of protein production using E. coli, the formation of insoluble inclusion bodies (IBs) poses a major challenge to harnessing E. coli as a host of choice. Furthermore, the complicated processing steps associated with IB extraction, solubilisation of IBs and refolding of the solubilised-denatured IB proteins, which are typically encountered in the traditional IB processing flowsheet, significantly compromise process economics of IB-route protein production using E. coli. In this paper, many recent advances in innovative IB processing technologies are reviewed with a special emphasis on their potential to contribute to process intensification, which is critical to achieve better process economics in the IB-route recombinant protein production using E. coli. © 2005 Published by Elsevier Ltd.|
|dc.contributor.department||CHEMICAL & BIOMOLECULAR ENGINEERING|
|dc.description.sourcetitle||Chemical Engineering Science|
|Appears in Collections:||Staff Publications|
Show simple item record
Files in This Item:
There are no files associated with this item.
checked on May 19, 2018
WEB OF SCIENCETM
checked on Oct 5, 2021
checked on Dec 2, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.