Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/64491
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dc.titleRecent advances in biomolecular process intensification
dc.contributor.authorChoe, W.-S.
dc.contributor.authorNian, R.
dc.contributor.authorLai, W.-B.
dc.date.accessioned2014-06-17T07:47:52Z
dc.date.available2014-06-17T07:47:52Z
dc.date.issued2006
dc.identifier.citationChoe, W.-S., Nian, R., Lai, W.-B. (2006). Recent advances in biomolecular process intensification. Chemical Engineering Science 61 (3) : 886-906. ScholarBank@NUS Repository.
dc.identifier.issn00092509
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64491
dc.description.abstractEscherichia coli has been the most popular cellular biofactory for the production of many biomolecules including recombinant proteins whose bioactivities are not dependent on complicated post-translational modifications such as glycosylation. Despite many advantages of protein production using E. coli, the formation of insoluble inclusion bodies (IBs) poses a major challenge to harnessing E. coli as a host of choice. Furthermore, the complicated processing steps associated with IB extraction, solubilisation of IBs and refolding of the solubilised-denatured IB proteins, which are typically encountered in the traditional IB processing flowsheet, significantly compromise process economics of IB-route protein production using E. coli. In this paper, many recent advances in innovative IB processing technologies are reviewed with a special emphasis on their potential to contribute to process intensification, which is critical to achieve better process economics in the IB-route recombinant protein production using E. coli. © 2005 Published by Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ces.2005.07.032
dc.sourceScopus
dc.subjectE. coli
dc.subjectInclusion body
dc.subjectProcess intensification
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.sourcetitleChemical Engineering Science
dc.description.volume61
dc.description.issue3
dc.description.page886-906
dc.description.codenCESCA
dc.identifier.isiut000233814600003
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