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Title: Recent advances in biomolecular process intensification
Authors: Choe, W.-S. 
Nian, R.
Lai, W.-B.
Keywords: E. coli
Inclusion body
Process intensification
Issue Date: 2006
Citation: Choe, W.-S., Nian, R., Lai, W.-B. (2006). Recent advances in biomolecular process intensification. Chemical Engineering Science 61 (3) : 886-906. ScholarBank@NUS Repository.
Abstract: Escherichia coli has been the most popular cellular biofactory for the production of many biomolecules including recombinant proteins whose bioactivities are not dependent on complicated post-translational modifications such as glycosylation. Despite many advantages of protein production using E. coli, the formation of insoluble inclusion bodies (IBs) poses a major challenge to harnessing E. coli as a host of choice. Furthermore, the complicated processing steps associated with IB extraction, solubilisation of IBs and refolding of the solubilised-denatured IB proteins, which are typically encountered in the traditional IB processing flowsheet, significantly compromise process economics of IB-route protein production using E. coli. In this paper, many recent advances in innovative IB processing technologies are reviewed with a special emphasis on their potential to contribute to process intensification, which is critical to achieve better process economics in the IB-route recombinant protein production using E. coli. © 2005 Published by Elsevier Ltd.
Source Title: Chemical Engineering Science
ISSN: 00092509
Appears in Collections:Staff Publications

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