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Title: In vitro antibacterial and cytotoxicity assay of multilayered polyelectrolyte-functionalized stainless steel
Authors: Shi, Z. 
Neoh, K.G. 
Zhong, S.P. 
Yung, L.Y.L. 
Kang, E.T. 
Wang, W.
Keywords: Antibacterial
Polyelectrolyte multilayers
Stainless steel
Surface functionalization
Issue Date: Mar-2006
Citation: Shi, Z., Neoh, K.G., Zhong, S.P., Yung, L.Y.L., Kang, E.T., Wang, W. (2006-03). In vitro antibacterial and cytotoxicity assay of multilayered polyelectrolyte-functionalized stainless steel. Journal of Biomedical Materials Research - Part A 76 (4) : 826-834. ScholarBank@NUS Repository.
Abstract: Infection of implanted materials by bacteria constitutes one of the most serious complications following prosthetic and implant surgery. In the present study, a new strategy for confering stainless steel with antibacterial property via the alternate deposition of quaternized polyethylenimine (PEI) or quaternized polyethylenimine-silver complex and poly(acrylic acid) (PAA) was investigated. The success of the deposition of the polyelectrolyte multilayers (PEM) and its chemical nature was investigated by static water contact angle and X-ray photoelectron spectroscopy (XPS), respectively. The antibacterial activity was assessed using Escherichia coli (E. coli, a gram-negative bacterium) and Staphylococcus aureus (S. aureus, a gram-positive bacterium). The inhibition of E. coli and S aureus growth on the surface of functionalized films was clearly shown using the LIVE/ DEAD Baclight bacterial viability kits and fluorescence microscopy. The cytotoxicity of the PEM to mammalian cells, evaluated by the MTT assay, was shown to be minimal and long-term antibacterial efficacy can be maintained. These results indicate new possibilities for the use of such easily built and functionalized architectures for the functionalization of surfaces of implanted medical devices. © 2005 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
ISSN: 15493296
DOI: 10.1002/jbm.a.30597
Appears in Collections:Staff Publications

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