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Title: Antibody-free detection of human chorionic gonadotropin by use of liquid crystals
Authors: Ding, X.
Yang, K.-L. 
Issue Date: 19-Nov-2013
Citation: Ding, X., Yang, K.-L. (2013-11-19). Antibody-free detection of human chorionic gonadotropin by use of liquid crystals. Analytical Chemistry 85 (22) : 10710-10716. ScholarBank@NUS Repository.
Abstract: Human chorionic gonadotropin (hCG) is an important biomarker for the diagnosis of pregnancy and cancers. In this study, we report an antibody-free and label-free mechanism for detecting hCG. To replace enzyme-labeled antibodies, we use a short oligopeptide as an hCG receptor to bind hCG. The short oligopeptide sequence, (N-)PPLRINRHILTR(-C), is identified after 5 rounds of screening by use of a phage library. After binding, liquid crystal (LC) is used to transduce the binding event into optical signals. The captured hCG can disrupt a thin layer (∼6 μm) of LC covered on the surface. Depending on the initial concentration of hCG, LC gives distinct optical signals visible to the naked eye. The limit of detection (LOD) for this method is approximately 1 IU/mL (2 nM) in both phosphate-buffered saline and urine samples, and only 0.6 μL of hCG solution is required. This means that as little as 45.5 pg of hCG can be detected by this method. Compared to other detection methods for hCG, this detection method does not require the use of antibody and is label-free. It has the potential to become a portable diagnostic kit for hCG. © 2013 American Chemical Society.
Source Title: Analytical Chemistry
ISSN: 00032700
DOI: 10.1021/ac400732n
Appears in Collections:Staff Publications

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