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Title: (Carboxymethyl)chitosan-modified superparamagnetic iron oxide nanoparticles for magnetic resonance imaging of stem cells
Authors: Shi, Z. 
Neoh, K.G. 
Kang, E.T. 
Shuter, B.
Wang, S.-C.
Poh, C.
Wang, W.
Keywords: (carboxymethyl)chitosan
magnetic resonance imaging
mesenchymal stem cell
superparamagnetic iron oxide nanoparticles
Issue Date: 25-Feb-2009
Citation: Shi, Z., Neoh, K.G., Kang, E.T., Shuter, B., Wang, S.-C., Poh, C., Wang, W. (2009-02-25). (Carboxymethyl)chitosan-modified superparamagnetic iron oxide nanoparticles for magnetic resonance imaging of stem cells. ACS Applied Materials and Interfaces 1 (2) : 328-335. ScholarBank@NUS Repository.
Abstract: Magnetic resonance imaging (MRI) is emerging as a powerful tool for in vivo noninvasive tracking of magnetically labeled stem cells. In this work, we present an efficient cell-labeling approach using (carboxymethyl)chitosan- modified superparamagnetic iron oxide nanoparticles (CMCS-SPIONs) as contrast agent in MRI. The CMCS-SPIONs were prepared by conjugating (carboxymethyl) chitosan to (3-aminopropyl)trimethoxysilane-treated SPIONs. These nanoparticles were internalized into human mesenchymal stem cells (hMSCs) via endocytosis as confirmed by Prussian Blue staining and electron microscopy investigation and quantified by inductively coupled plasma mass spectrometry. A MTT assay of the labeled cells showed that CMCS-SPIONs did not possess significant cytotoxicity. In addition, the osteogenic and adipogenic differentiations of the hMSCs were not influenced by the labeling process. The in vitro detection threshold of cells after incubation with 0.05 mg/mL of CMCS-SPIONs for 24 h was estimated to be about 40 cells. The results from this study indicate that the biocompatible CMCS-SPIONs show promise for use with MRI in visualizing hMSCs. © 2008 American Chemical Society.
Source Title: ACS Applied Materials and Interfaces
ISSN: 19448244
DOI: 10.1021/am8000538
Appears in Collections:Staff Publications

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