Please use this identifier to cite or link to this item:
Title: Kinetics of cell death of frozen-thawed human embryonic stem cell colonies is reversibly slowed down by exposure to low temperature
Authors: Heng, B.C. 
Ye, C.P.
Liu, H. 
Toh, W.S. 
Rufaihah, A.J. 
Cao, T. 
Keywords: Cell death
Stem cells
Issue Date: Nov-2006
Citation: Heng, B.C., Ye, C.P., Liu, H., Toh, W.S., Rufaihah, A.J., Cao, T. (2006-11). Kinetics of cell death of frozen-thawed human embryonic stem cell colonies is reversibly slowed down by exposure to low temperature. Zygote 14 (4) : 341-348. ScholarBank@NUS Repository.
Abstract: A major challenge in the widespread application of hES (human embryonic stem) cells in clinical therapy and basic scientific research is the development of efficient cryopreservation protocols. Conventional slow-cooling protocols utilizing standard cryoprotectant concentrations i.e. 10% (v/v) DMSO, yield extremely low survival rates of less than 5% as reported by previous studies. This study characterized cell death in frozen-thawed hES colonies that were cryopreserved under standard conditions. Surprisingly, our results showed that immediately after post-thaw washing, the overwhelming majority of hES cells were viable (approximately 98%), as assessed by the trypan blue exclusion test. However, when the freshly thawed hES colonies were placed in a 37°C incubator, there was a gradual reduction in cell viability over time. The kinetics of cell death was drastically slowed down by keeping the freshly thawed hES colonies at 4°C, with more than 90% of cells remaining viable after 90 min of incubation at 4°C. This effect was reversible upon re-exposing the cells to physiological temperatures. The vast majority of low temperature-exposed hES colonies gradually underwent cell death upon incubation for a further 90 min at 37°C. Hence, our observations would strongly suggest involvement of a self-induced apoptotic mechanism, as opposed to cellular necrosis arising from cryoinjury. © 2006 Cambridge University Press.
Source Title: Zygote
ISSN: 09671994
DOI: 10.1017/S0967199406003893
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Jul 18, 2019


checked on Jul 10, 2019

Page view(s)

checked on Jun 22, 2019

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.