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Title: Comparison of Human alveolar osteoblasts cultured on polymer-ceramic composite scaffolds and tissue culture plates
Authors: Yefang, Z. 
Hutmacher, D.W. 
Varawan, S.-L. 
Meng, L.T. 
Keywords: bone engineering
calcium phosphate
human alveolar osteoblasts
Issue Date: Feb-2007
Citation: Yefang, Z., Hutmacher, D.W., Varawan, S.-L., Meng, L.T. (2007-02). Comparison of Human alveolar osteoblasts cultured on polymer-ceramic composite scaffolds and tissue culture plates. International Journal of Oral and Maxillofacial Surgery 36 (2) : 137-145. ScholarBank@NUS Repository.
Abstract: The effects of medical grade polycaprolactone-tricalcium phosphate (mPCL-TCP) (80:20) scaffolds on primary human alveolar osteoblasts (AOs) were compared with standard tissue-culture plates. Of the seeded AOs, 70% adhered to and proliferated on the scaffold surface and within open and interconnected pores; they formed multi-layered sheets and collagen fibers with uniform distribution within 28 days. Elevation of alkaline phosphatase activity occurred in scaffold-cell constructs independent of osteogenic induction. AO proliferation rate increased and significant decrease in calcium concentration of the medium for both scaffolds and plates under induction conditions were seen. mPCL-TCP scaffolds significantly influenced the AO expression pattern of osterix and osteocalcin (OCN). Osteogenic induction down-regulated OCN at both RNA and protein level on scaffolds (3D) by day 7, and up-regulated OCN in cell-culture plates (2D) by day 14, but OCN levels on scaffolds were higher than on cell-culture plates. Immunocytochemical signals for type I collagen, osteopontin and osteocalcin were detected at the outer parts of scaffold-cell constructs. More mineral nodules were found in induced than in non-induced constructs. Only induced 2D cultures showed nodule formation. mPCL-TCP scaffolds appear to stimulate osteogenesis in vitro by activating a cellular response in AO's to form mineralized tissue. There is a fundamental difference between culturing AOs on 2D and 3D environments that should be considered when studying osteogenesis in vitro. © 2006.
Source Title: International Journal of Oral and Maxillofacial Surgery
ISSN: 09015027
DOI: 10.1016/j.ijom.2006.08.012
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