Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ijom.2006.08.012
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dc.titleComparison of Human alveolar osteoblasts cultured on polymer-ceramic composite scaffolds and tissue culture plates
dc.contributor.authorYefang, Z.
dc.contributor.authorHutmacher, D.W.
dc.contributor.authorVarawan, S.-L.
dc.contributor.authorMeng, L.T.
dc.date.accessioned2014-05-16T04:58:20Z
dc.date.available2014-05-16T04:58:20Z
dc.date.issued2007-02
dc.identifier.citationYefang, Z., Hutmacher, D.W., Varawan, S.-L., Meng, L.T. (2007-02). Comparison of Human alveolar osteoblasts cultured on polymer-ceramic composite scaffolds and tissue culture plates. International Journal of Oral and Maxillofacial Surgery 36 (2) : 137-145. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ijom.2006.08.012
dc.identifier.issn09015027
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/52520
dc.description.abstractThe effects of medical grade polycaprolactone-tricalcium phosphate (mPCL-TCP) (80:20) scaffolds on primary human alveolar osteoblasts (AOs) were compared with standard tissue-culture plates. Of the seeded AOs, 70% adhered to and proliferated on the scaffold surface and within open and interconnected pores; they formed multi-layered sheets and collagen fibers with uniform distribution within 28 days. Elevation of alkaline phosphatase activity occurred in scaffold-cell constructs independent of osteogenic induction. AO proliferation rate increased and significant decrease in calcium concentration of the medium for both scaffolds and plates under induction conditions were seen. mPCL-TCP scaffolds significantly influenced the AO expression pattern of osterix and osteocalcin (OCN). Osteogenic induction down-regulated OCN at both RNA and protein level on scaffolds (3D) by day 7, and up-regulated OCN in cell-culture plates (2D) by day 14, but OCN levels on scaffolds were higher than on cell-culture plates. Immunocytochemical signals for type I collagen, osteopontin and osteocalcin were detected at the outer parts of scaffold-cell constructs. More mineral nodules were found in induced than in non-induced constructs. Only induced 2D cultures showed nodule formation. mPCL-TCP scaffolds appear to stimulate osteogenesis in vitro by activating a cellular response in AO's to form mineralized tissue. There is a fundamental difference between culturing AOs on 2D and 3D environments that should be considered when studying osteogenesis in vitro. © 2006.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ijom.2006.08.012
dc.sourceScopus
dc.subjectbone engineering
dc.subjectcalcium phosphate
dc.subjectcomposites
dc.subjecthuman alveolar osteoblasts
dc.subjectosteogenesis
dc.subjectscaffolds
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentRESTORATIVE DENTISTRY
dc.description.doi10.1016/j.ijom.2006.08.012
dc.description.sourcetitleInternational Journal of Oral and Maxillofacial Surgery
dc.description.volume36
dc.description.issue2
dc.description.page137-145
dc.description.codenIJOSE
dc.identifier.isiut000244591500007
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