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Title: Biomimetic surface modification of titanium surfaces for early cell capture by advanced electrospinning
Authors: Ravichandran, R.
Ng, C.C.H. 
Liao, S.
Pliszka, D. 
Raghunath, M. 
Ramakrishna, S. 
Chan, C.K. 
Issue Date: Feb-2012
Citation: Ravichandran, R., Ng, C.C.H., Liao, S., Pliszka, D., Raghunath, M., Ramakrishna, S., Chan, C.K. (2012-02). Biomimetic surface modification of titanium surfaces for early cell capture by advanced electrospinning. Biomedical Materials 7 (1) : -. ScholarBank@NUS Repository.
Abstract: The time required for osseointegration with a metal implant having a smooth surface ranges from three to six months. We hypothesized that biomimetic coating surfaces with poly(lactic-co-glycolic acid) (PLGA)/collagen fibers and nano-hydroxyapatite (n-HA) on the implant would enhance the adhesion of mesenchymal stem cells. Therefore, this surface modification of dental and bone implants might enhance the process of osseointegration. In this study, we coated PLGA or PLGA/collagen (50:50 w/w ratio) fiber on Ti disks by modified electrospinning for 5 s to 2 min; after that, we further deposited n-HA on the fibers. PLGA fibers of fiber diameter 0.957 ± 0.357 μm had a contact angle of 9.9 ± 0.3° and PLGA/collagen fibers of fiber diameter 0.378 ± 0.068 μm had a contact angle of 0°. Upon n-HA incorporation, all the fibers had a contact angle of 0° owing to the hydrophilic nature of n-HA biomolecule. The cell attachment efficiency was tested on all the scaffolds for different intervals of time (10, 20, 30 and 60 min). The alkaline phosphatase activity, cell proliferation and mineralization were analyzed on all the implant surfaces on days 7, 14 and 21. Results of the cell adhesion study indicated that the cell adhesion was maximum on the implant surface coated with PLGA/collagen fibers deposited with n-HA compared to the other scaffolds. Within a short span of 60 min, 75% of the cells adhered onto the mineralized PLGA/collagen fibers. Similarly by day 21, the rate of cell proliferation was significantly higher (p ≤ 0.05) on the mineralized PLGA/collagen fibers owing to enhanced cell adhesion on these fibers. This enhanced initial cell adhesion favored higher cell proliferation, differentiation and mineralization on the implant surface coated with mineralized PLGA/collagen fibers. © 2012 IOP Publishing Ltd.
Source Title: Biomedical Materials
ISSN: 17486041
DOI: 10.1088/1748-6041/7/1/015001
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