Please use this identifier to cite or link to this item:
|Title:||Combined effects of TGFβ1 and BMP2 in serum-free chondrogenic differentiation of mesenchymal stem cells induced hyaline-like cartilage formation||Authors:||Toh, W.S.
|Issue Date:||2005||Citation:||Toh, W.S., Liu, H., Heng, B.C., Rufaihah, A.J., Ye, C.P., Cao, T. (2005). Combined effects of TGFβ1 and BMP2 in serum-free chondrogenic differentiation of mesenchymal stem cells induced hyaline-like cartilage formation. Growth Factors 23 (4) : 313-321. ScholarBank@NUS Repository. https://doi.org/10.1080/08977190500252763||Abstract:||This study investigated the effects of TGFβ1, BMP2 or a combination of both on the chondrogenic differentiation of mesenchymal stem cells (MSCs) in a serum-free micromass culture system in vitro. Putative MSCs harvested from the iliac crest of 4-5 month old New Zealand White Rabbits were expanded and cultured in three-dimensional high density micromass aggregate cultures containing TGFβ1, BMP2 or a combination of both, in the absence of serum. After 14-20 days of culture, chondrogenic differentiation of the MSCs was assayed by toluidine blue staining, immunohistochemistry and semi-quantitative RT-RCR of type I collagen (CI) and type II collagen (CII). Quantitative measurements of cell proliferation and sulfated glycosaminoglycan (s-GAG) were also carried out to assess the growth rate and matrix deposition of the cultured aggregates. Both immunohistochemical staining and semi-quantitative RT-PCR showed that the combination of BMP2 and TGFβ1 resulted in a marked enhancement of collagen II synthesis, with minimal collagen I expression, which would suggest hyaline-like cartilage formation. Additionally, BMP2 and TGFβ1 had a synergistic effect on matrix proteoglycan deposition, as assessed by metachromatic toluidine blue staining. This is consistent with the quantitative measurement of glycosaminoglycans, whereby a significant increase in GAG/DNA was noted in the co-treatment group. Hence, it can be concluded that the combination of BMP2 and TGFβ1 has a synergistic effect on the differentiation of MSC into hyaline-like cartilage tissue. © 2005 Taylor & Francis.||Source Title:||Growth Factors||URI:||http://scholarbank.nus.edu.sg/handle/10635/47060||ISSN:||08977194||DOI:||10.1080/08977190500252763|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 16, 2019
WEB OF SCIENCETM
checked on Jul 16, 2019
checked on Jul 21, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.