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Title: Expression and secretion of the vascular cell adhesion molecule-1 in human placenta and its decrease in fetal growth restriction
Authors: Rajashekhar, G. 
Loganath, A. 
Roy, A.C. 
Wong, Y.C. 
Keywords: Angiogenesis
Explant culture
Issue Date: 2003
Citation: Rajashekhar, G., Loganath, A., Roy, A.C., Wong, Y.C. (2003). Expression and secretion of the vascular cell adhesion molecule-1 in human placenta and its decrease in fetal growth restriction. Journal of the Society for Gynecologic Investigation 10 (6) : 352-360. ScholarBank@NUS Repository.
Abstract: Objective: The vascular cell adhesion molecule-1 (VCAM-1) is a member of the immunoglobulin gene superfamily and is expressed principally on endothelial cells. The present study was undertaken to compare the expression and secretion of VCAM-1 from normal pregnancies and those complicated by fetal growth restriction (FGR). Methods: Placentas from first-trimester (FT) (n = 17), normal term (n = 19), and FGR (n = 16) patients were collected immediately after elective cesarean delivery. VCAM-1 mRNA expression profiles by reverse transcriptase polymerase chain reaction, protein levels by enzyme-linked immunosorbent assay using explant culture in vitro, and its cellular localization by confocal microscopy were compared between FGR and normal placentas. Results: Functionally active placental explants were used to detect immunoreactive VCAM-1 in conditioned media of all the samples from the three groups. The mean levels of VCAM-1 produced by FT villi were found to be 1.9-, 1.7-, and 1.5-fold higher (P < .02) than term villi at 24, 48, and 72 hours of culture, respectively. Conversely, the respective mean levels of VCAM-1 produced by FGR placental villi were 2.3-, 2.5-, and 2.0-fold lower than levels of the normal term placental villi (P < .05). The secretion profiles of VCAM-1 from FT, term, and FGR villi correlated well with the mRNA levels; the amount secreted in FT villi was twice that of term villi. Moreover, mRNA transcripts from FGR pregnancies showed significantly decreased expression, in conformity with explant results. Conclusions: The presence of VCAM-1 in placental villi and down-regulation of its production at term indicate that VCAM-1 production is specific to developmental stage. The decreased VCAM-1 expression in FGR pregnancy could be attributed to the uteroplacental deficiency that is characteristic of this condition. Copyright © 2003 by the Society for Gynecologic Investigation.
Source Title: Journal of the Society for Gynecologic Investigation
ISSN: 10715576
DOI: 10.1016/S1071-5576(03)00121-7
Appears in Collections:Staff Publications

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