Please use this identifier to cite or link to this item: https://doi.org/10.1016/S1071-5576(03)00121-7
DC FieldValue
dc.titleExpression and secretion of the vascular cell adhesion molecule-1 in human placenta and its decrease in fetal growth restriction
dc.contributor.authorRajashekhar, G.
dc.contributor.authorLoganath, A.
dc.contributor.authorRoy, A.C.
dc.contributor.authorWong, Y.C.
dc.date.accessioned2013-04-10T02:58:46Z
dc.date.available2013-04-10T02:58:46Z
dc.date.issued2003
dc.identifier.citationRajashekhar, G., Loganath, A., Roy, A.C., Wong, Y.C. (2003). Expression and secretion of the vascular cell adhesion molecule-1 in human placenta and its decrease in fetal growth restriction. Journal of the Society for Gynecologic Investigation 10 (6) : 352-360. ScholarBank@NUS Repository. https://doi.org/10.1016/S1071-5576(03)00121-7
dc.identifier.issn10715576
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/36916
dc.description.abstractObjective: The vascular cell adhesion molecule-1 (VCAM-1) is a member of the immunoglobulin gene superfamily and is expressed principally on endothelial cells. The present study was undertaken to compare the expression and secretion of VCAM-1 from normal pregnancies and those complicated by fetal growth restriction (FGR). Methods: Placentas from first-trimester (FT) (n = 17), normal term (n = 19), and FGR (n = 16) patients were collected immediately after elective cesarean delivery. VCAM-1 mRNA expression profiles by reverse transcriptase polymerase chain reaction, protein levels by enzyme-linked immunosorbent assay using explant culture in vitro, and its cellular localization by confocal microscopy were compared between FGR and normal placentas. Results: Functionally active placental explants were used to detect immunoreactive VCAM-1 in conditioned media of all the samples from the three groups. The mean levels of VCAM-1 produced by FT villi were found to be 1.9-, 1.7-, and 1.5-fold higher (P < .02) than term villi at 24, 48, and 72 hours of culture, respectively. Conversely, the respective mean levels of VCAM-1 produced by FGR placental villi were 2.3-, 2.5-, and 2.0-fold lower than levels of the normal term placental villi (P < .05). The secretion profiles of VCAM-1 from FT, term, and FGR villi correlated well with the mRNA levels; the amount secreted in FT villi was twice that of term villi. Moreover, mRNA transcripts from FGR pregnancies showed significantly decreased expression, in conformity with explant results. Conclusions: The presence of VCAM-1 in placental villi and down-regulation of its production at term indicate that VCAM-1 production is specific to developmental stage. The decreased VCAM-1 expression in FGR pregnancy could be attributed to the uteroplacental deficiency that is characteristic of this condition. Copyright © 2003 by the Society for Gynecologic Investigation.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S1071-5576(03)00121-7
dc.sourceScopus
dc.subjectAngiogenesis
dc.subjectExplant culture
dc.subjectRT-PCR
dc.subjectTrophoblast
dc.typeArticle
dc.contributor.departmentOBSTETRICS & GYNAECOLOGY
dc.description.doi10.1016/S1071-5576(03)00121-7
dc.description.sourcetitleJournal of the Society for Gynecologic Investigation
dc.description.volume10
dc.description.issue6
dc.description.page352-360
dc.description.codenJSGIE
dc.identifier.isiut000185402000005
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

8
checked on Oct 23, 2019

WEB OF SCIENCETM
Citations

8
checked on Oct 23, 2019

Page view(s)

112
checked on Oct 14, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.