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https://scholarbank.nus.edu.sg/handle/10635/34828
Title: | A NOVEL GENERATION OF CLONED HORSESHOE CRAB RECOMBINANT FACTOR C FOR DETECTION AND REMOVAL OF ENDOTOXIN | Authors: | DING, JEAK LING HO, BOW |
Issue Date: | 5-Jul-2000 | Citation: | DING, JEAK LING,HO, BOW (2000-07-05). A NOVEL GENERATION OF CLONED HORSESHOE CRAB RECOMBINANT FACTOR C FOR DETECTION AND REMOVAL OF ENDOTOXIN. ScholarBank@NUS Repository. | Abstract: | The horseshoe crab, i(Carcinoscropius rotundicauda) Factor C cDNA (CrFC21) has been cloned into a shuttle baculoviral vector. The recombinant baculoviral DNA was then transfected into the insect cells for expression of recombinant Factor C. Recombinant Factor C was found to be immunoreactive and is capable of binding both free and bound/immobilized lipid A. It is enzymatically active when triggered by LPS. The rFC is probably of the two-chain form, being cleaved into the heavy and light chains after activation by Gram negative bacterial endotoxin. As low as 0.01 pg (0.001 ng/ml) of LPS was detectable by the rFC, thus, indicating its potentials as a novel generation of "limulus amoebocyte lysate". | URI: | http://scholarbank.nus.edu.sg/handle/10635/34828 |
Appears in Collections: | Staff Publications |
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