Please use this identifier to cite or link to this item: http://scholarbank.nus.edu.sg/handle/10635/34828
Title: A NOVEL GENERATION OF CLONED HORSESHOE CRAB RECOMBINANT FACTOR C FOR DETECTION AND REMOVAL OF ENDOTOXIN
Authors: DING, JEAK LING 
HO, BOW 
Issue Date: 5-Jul-2000
Source: DING, JEAK LING,HO, BOW (2000-07-05). A NOVEL GENERATION OF CLONED HORSESHOE CRAB RECOMBINANT FACTOR C FOR DETECTION AND REMOVAL OF ENDOTOXIN. ScholarBank@NUS Repository.
Abstract: The horseshoe crab, i(Carcinoscropius rotundicauda) Factor C cDNA (CrFC21) has been cloned into a shuttle baculoviral vector. The recombinant baculoviral DNA was then transfected into the insect cells for expression of recombinant Factor C. Recombinant Factor C was found to be immunoreactive and is capable of binding both free and bound/immobilized lipid A. It is enzymatically active when triggered by LPS. The rFC is probably of the two-chain form, being cleaved into the heavy and light chains after activation by Gram negative bacterial endotoxin. As low as 0.01 pg (0.001 ng/ml) of LPS was detectable by the rFC, thus, indicating its potentials as a novel generation of "limulus amoebocyte lysate".
URI: http://scholarbank.nus.edu.sg/handle/10635/34828
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