Please use this identifier to cite or link to this item:
|Title:||High-performance affinity capture-removal of bacterial pyrogen from solutions||Authors:||Ding, J.L.
|Issue Date:||2001||Citation:||Ding, J.L., Zhu, Y., Ho, B. (2001). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-4347(01)00227-4||Abstract:||Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.||Source Title:||Journal of Chromatography B: Biomedical Sciences and Applications||URI:||http://scholarbank.nus.edu.sg/handle/10635/31474||ISSN:||13872273||DOI:||10.1016/S0378-4347(01)00227-4|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 24, 2020
WEB OF SCIENCETM
checked on Sep 17, 2020
checked on Sep 22, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.