Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-4347(01)00227-4
Title: High-performance affinity capture-removal of bacterial pyrogen from solutions
Authors: Ding, J.L. 
Zhu, Y.
Ho, B. 
Keywords: Affinity adsorption
Endotoxins
Lipopolysaccharides
Pyrogen
Issue Date: 2001
Citation: Ding, J.L., Zhu, Y., Ho, B. (2001). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-4347(01)00227-4
Abstract: Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.
Source Title: Journal of Chromatography B: Biomedical Sciences and Applications
URI: http://scholarbank.nus.edu.sg/handle/10635/31474
ISSN: 13872273
DOI: 10.1016/S0378-4347(01)00227-4
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

28
checked on Sep 20, 2018

WEB OF SCIENCETM
Citations

24
checked on Sep 5, 2018

Page view(s)

129
checked on Aug 17, 2018

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.