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|Title:||High-performance affinity capture-removal of bacterial pyrogen from solutions|
|Authors:||Ding, J.L. |
|Source:||Ding, J.L., Zhu, Y., Ho, B. (2001). High-performance affinity capture-removal of bacterial pyrogen from solutions. Journal of Chromatography B: Biomedical Sciences and Applications 759 (2) : 237-246. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-4347(01)00227-4|
|Abstract:||Synthetic peptide S3Δ has high affinity for bacterial endotoxin or lipopolysaccharide (LPS). Under tested conditions of pH 5-9 and 0-0.4 M NaCl, the affinity constant, KD ranged from 2·10-6 to 2·10-9 M-1. A novel affinity matrix based on peptide S3Δ was developed for removal of LPS from solutions such as: water; buffers with a wide range of ionic strength and pH; medium for cell culture; and protein solutions under optimized conditions. At a starting LPS of ≈100 EU/ml, a post-purification level below 0.005 EU/ml was achieved. © 2001 Elsevier Science B.V.|
|Source Title:||Journal of Chromatography B: Biomedical Sciences and Applications|
|Appears in Collections:||Staff Publications|
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