Please use this identifier to cite or link to this item:
|Title:||Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2||Authors:||Fong, K.P.Y.
cis-Benzene dihydrodiol dehydrogenase
Pseudomonas putida ML2
Type III alcohol dehydrogenase
|Issue Date:||1999||Citation:||Fong, K.P.Y., Tan, H.-M. (1999). Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2. FEBS Letters 451 (1) : 5-9. ScholarBank@NUS Repository. https://doi.org/10.1016/S0014-5793(99)00520-7||Abstract:||A second and novel cis-benzene dihydrodiol dehydrogenase which is able to dehydrogenate a range of cis-dihydrodiols and other vicinal alcohols has been purified from Pseudomonas putida ML2. The enzyme is a tetramer of a polypeptide of 39 kDa in molecular mass and has a pH optimum of 9.0. Despite having a primary structure that has significant similarity to glycerol dehydrogenases, the k(cat)/K(m) value of the enzyme for cis-benzene dihydrodiol is 4300-fold higher compared to glycerol. The apparent K(m) values of the enzyme for cis-benzene dihydrodiol and glycerol are 0.01 mM and 46 mM, respectively, and 0.22 mM for NAD+. Copyright (C) 1999 Federation of European Biochemical Societies.||Source Title:||FEBS Letters||URI:||http://scholarbank.nus.edu.sg/handle/10635/31313||ISSN:||00145793||DOI:||10.1016/S0014-5793(99)00520-7|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jul 5, 2020
WEB OF SCIENCETM
checked on Jun 26, 2020
checked on Jun 30, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.