Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0014-5793(99)00520-7
Title: Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2
Authors: Fong, K.P.Y.
Tan, H.-M. 
Keywords: Benzene catabolism
cis-Benzene dihydrodiol dehydrogenase
Pseudomonas putida ML2
Type III alcohol dehydrogenase
Issue Date: 1999
Source: Fong, K.P.Y., Tan, H.-M. (1999). Characterization of a novel cis-benzene dihydrodiol dehydrogenase from Pseudomonas putida ML2. FEBS Letters 451 (1) : 5-9. ScholarBank@NUS Repository. https://doi.org/10.1016/S0014-5793(99)00520-7
Abstract: A second and novel cis-benzene dihydrodiol dehydrogenase which is able to dehydrogenate a range of cis-dihydrodiols and other vicinal alcohols has been purified from Pseudomonas putida ML2. The enzyme is a tetramer of a polypeptide of 39 kDa in molecular mass and has a pH optimum of 9.0. Despite having a primary structure that has significant similarity to glycerol dehydrogenases, the k(cat)/K(m) value of the enzyme for cis-benzene dihydrodiol is 4300-fold higher compared to glycerol. The apparent K(m) values of the enzyme for cis-benzene dihydrodiol and glycerol are 0.01 mM and 46 mM, respectively, and 0.22 mM for NAD+. Copyright (C) 1999 Federation of European Biochemical Societies.
Source Title: FEBS Letters
URI: http://scholarbank.nus.edu.sg/handle/10635/31313
ISSN: 00145793
DOI: 10.1016/S0014-5793(99)00520-7
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