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|Title:||Expression of interleukin-18 by nasopharyngeal carcinoma cells: A factor that possibly initiates the massive leukocyte infiltration||Authors:||Chan, S.H.
|Issue Date:||2004||Citation:||Chan, S.H., Hu, H., Tang, K.F., Chua, Y.N., Lu, J., Feng, P., Chew, C.T. (2004). Expression of interleukin-18 by nasopharyngeal carcinoma cells: A factor that possibly initiates the massive leukocyte infiltration. Human Pathology 35 (6) : 722-728. ScholarBank@NUS Repository.||Abstract:||Interleukin-18 (IL-18) is a single-chain cytokine that is produced by various cells. With interleukin-12 (IL-12), it synergistically stimulates activated T cells and natural killer (NK) cells to produce interferon-γ (IFN-γ). Nasopharyngeal carcinoma (NPC) is the most common form of nasal and nasopharyngeal malignancy, and in NPC tumor tissues there is an intense leukocyte infiltration comprising predominantly T cells and macrophages. We previously showed an increased expression of IFN-γ in the infiltrating T cells. To identify the cells that provide IL-12 and IL-18 for stimulating the expression of IFN-γ in activated T cells, NPC cell lines CNE-2 and HK-1, as well as biopsies obtained from NPC and control individuals, were examined. CNE-2 and HK-1 cells were found to express messenger RNA encoding IL-18, but not IL-12. Secreted IL-18 was detected in the culture supernatant. Addition of a caspase-1 inhibitor decreased the secretion level, indicating that this IL-18 secretion was caspase-1 dependent. Moreover, the in vitro IL-18 production in NPC cell lines correlated with the NPC tumor cells in situ. NPC tumor cells in the biopsies produced IL-18, as detected by immunohistochemistry and immunofluorescent double staining. In contrast, IL-18 expression was not observed in the control biopsies. We suggest that IL-18 secreted by NPC tumor cells plays a role in initiating the leukocyte infiltration process. IL-18 stimulates T cells and NK cells to produce IFN-γ, which consequently activates macrophages and other immune cells to secrete chemokines to start a leukocyte recruitment cascade. © 2004 Elsevier Inc. All rights reserved.||Source Title:||Human Pathology||URI:||http://scholarbank.nus.edu.sg/handle/10635/31187||ISSN:||00468177|
|Appears in Collections:||Staff Publications|
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