Development of nanoporous alumina-based electromembrane system
CHEOW PUI SZE
CHEOW PUI SZE
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Abstract
A new type of membrane electrodes based on nanoporous alumina membranes has been developed. This thesis describes the use of the alumina membrane system for transport studies and separation of proteins, gold nanoparticles and oligonucleotides.A conductive nanoporous alumina membrane electrode was fabricated by physical deposition of conductive material on both sides of the membrane. Besides, surface modification of the alumina membranes which could produce surfaces with varied functionalities had been carried out.Single protein transport studies and protein separation were carried out using the alumina membrane under the influence of different applied potential across the membrane. Protein concentration increased in the receiver solution under favourable electrophoretic conditions. Besides, good separation resolution for BSA and Lys was achieved at pH 10 and using a polyethylene glycol-modified membrane. Alumina electromembrane system showed the ability to characterize gold nanoparticles into various different sizes under optimized conditions. Besides, the electrokinetic transports of oligonucleotides could be analysed by using the static system.
Keywords
Alumina, electromembrane, protein, nanoparticle, oligonucleotide, separation
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Date
2008-04-16
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Thesis